KMID : 1005420050070010061
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Journal of Cerebrovascular and Endovascular Neurosurgery 2005 Volume.7 No. 1 p.61 ~ p.68
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Histological and Functional Changes after Transplantation of Human Mesenchymal Stem Cell in the Ischemic Rat Model
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Kim Hyung-Suk
Song Ji-Young Kim Hyun-Soo Yun Hee-Un Lee Kyung-Bok Cho Kyung-Gi
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Abstract
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Objectives: Brain transplantation has emerged as an effective treatment for patients suffering from neurodegenerative diseases, including Parkinson¡¯s disease, Huntingtons disease and Stroke. We evaluated that cytokine inducted human mesenchymal stem cells (Ci-hMSCs) transplanted in brain differentiated into neural cells and improved neurological functions after stroke in rats.
Materials and Methods: In the adult female Sprague-Dawley rats, ischemic lesion was induced by transient MCA occlusion lasted for 2 hours. One day later, Ci-hMSCs carrying LacZ gene were implanted via tail vein. The animals were assessed for sensorymotor function and sacrificed for Immunohistochemical staining at 7, 14, 28, 56 days after transplantation.
Results: A large number of X-gal positive hMSCs were observed in the ischemic core and ischemic boundary zone. Some hMSCs were reactive for the astrocytic marker - glial fibrillary acidic protein (GFAP) and neuronal marker - neuronal nuclear antigen (NeuN). The ischemic rats that were transplanted with Ci-hMSCs exhibited better functional improvement than control groups and the rats with hMSCs, which was statistically significant.
Conclusion: The neuronal differentiation of CihMSCs suggested that transplantation of the Ci-hMSCs may provide the possibility of the clinical implication for cerebral stroke.
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KEYWORD
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Stroke, Brain transplantation, Human mesenchymal stem cells, Cytokine inducted human mesenchymal stem cells
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